Notes
- Unclassified reads were excluded
- Taxa with total reads number of 10 or lower were excluded
- Eukaryotes were excluded from the analyis
Import Exp data
MCM D6300
MCM D6331
FR D6323
Canis lupus
Homo sapiens
Merge
Filter Exp data
Filter samples based on total read count
Fix sample names
Fix sample names from ‘-’
Merge exp data with GS
Filter Taxa for Gold Standard
These are the taxa that were found in the experimental data
Add Gram Stain Info
Omit Eukaryotes
Zymo ONT V1-V9 FR vs Homo sapiens and Canis lupus
comparison
Parameters and Settings
Correlation
PERMANOVA model
Gram Stain level
Barplot of abundances

Phylum level
Barplot of abundacnes

MAASLIN2 - Number of Significantly Different Taxa Among FR,
H. sapiens and C. lupus

Barplot of Coefficients

Heatmap of Significant Associations (−log(qval) x
sign(coeff))

PERMANOVA
| primer |
2 |
0.2220239 |
0.1815434 |
2.661743 |
0.069 |
| Residual |
24 |
1.0009559 |
0.8184566 |
NA |
NA |
| Total |
26 |
1.2229798 |
1.0000000 |
NA |
NA |

Class level
Barplot of abundances

MAASLIN2 - Number of Significantly Different Taxa Among FR,
H. sapiens and C. lupus

Barplot of Coefficients

Heatmap of Significant Associations (−log(qval) x
sign(coeff))

PERMANOVA
| primer |
2 |
0.2551431 |
0.1460105 |
2.051694 |
0.047 |
| Residual |
24 |
1.4922872 |
0.8539895 |
NA |
NA |
| Total |
26 |
1.7474303 |
1.0000000 |
NA |
NA |

Order level
Barplot of abundances

MAASLIN2 - Number of Significantly Different Taxa Among FR,
H. sapiens and C. lupus

Barplot of Coefficients

Heatmap of Significant Associations (−log(qval) x
sign(coeff))
### PERMANOVA
| primer |
2 |
0.2509976 |
0.1403119 |
1.958551 |
0.056 |
| Residual |
24 |
1.5378566 |
0.8596881 |
NA |
NA |
| Total |
26 |
1.7888542 |
1.0000000 |
NA |
NA |

Family level
Barplot of abundances

MAASLIN2 - Number of Significantly Different Taxa Among FR,
H. sapiens and C. lupus

Barplot of Coefficients

Heatmap of Significant Associations (−log(qval) x
sign(coeff))

PERMANOVA
| primer |
2 |
0.3283757 |
0.1074244 |
1.444239 |
0.005 |
| Residual |
24 |
2.7284326 |
0.8925756 |
NA |
NA |
| Total |
26 |
3.0568083 |
1.0000000 |
NA |
NA |

Genus level
Barplot of abundances

MAASLIN2 - Number of Significantly Different Taxa Among FR,
H. sapiens and C. lupus

Barplot of Coefficients

Heatmap of Significant Associations (−log(qval) x
sign(coeff))

PERMANOVA
| primer |
2 |
0.2487811 |
0.0619096 |
0.7919443 |
0.014 |
| Residual |
24 |
3.7696752 |
0.9380904 |
NA |
NA |
| Total |
26 |
4.0184563 |
1.0000000 |
NA |
NA |

Species level
Barplot of abundances

MAASLIN2 - Number of Significantly Different Taxa Among FR,
H. sapiens and C. lupus

Barplot of Coefficients

Heatmap of Significant Associations (−log(qval) x
sign(coeff))
